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OIL AND GAS
An oil-eating bacterium that can help clean up pollution and spills
by Staff Writers
Montreal, Canada (SPX) Apr 10, 2018

Professor Satinder Kaur Brar at the Environmental Biotechnology Laboratory

From pipelines to tankers, oil spills and their impact on the environment are a source of concern. These disasters occur on a regular basis, leading to messy decontamination challenges that require massive investments of time and resources. But however widespread and serious the damage may be, the solution could be microscopic - Alcanivorax borkumensis - a bacterium that feeds on hydrocarbons.

Professor Satinder Kaur Brar and her team at INRS have conducted laboratory tests that show the effectiveness of enzymes produced by the bacterium in degrading petroleum products in soil and water. Their results offer hope for a simple, effective, and eco-friendly method of decontaminating water and soil at oil sites.

In recent years, researchers have sequenced the genomes of thousands of bacteria from various sources. Research associate Dr.Tarek Rouissi poured over "technical data sheets" for many bacterial strains with the aim of finding the perfect candidate for a dirty job: cleaning up oil spills. He focused on the enzymes they produce and the conditions in which they evolve.

A. borkumensis, a non-pathogenic marine bacterium piqued his curiosity. The microorganism's genome contains the codes of a number of interesting enzymes and it is classified as "hydrocarbonoclastic" - i.e., as a bacterium that uses hydrocarbons as a source of energy.

A. borkumensis is present in all oceans and drifts with the current, multiplying rapidly in areas where the concentration of oil compounds is high, which partly explains the natural degradation observed after some spills. But its remedial potential had not been assessed.

"I had a hunch," Rouissi said, "and the characterization of the enzymes produced by the bacterium seems to have proven me right!" A. borkumensis boasts an impressive set of tools: during its evolution, it has accumulated a range of very specific enzymes that degrade almost everything found in oil.

Among these enzymes, the bacteria'shydroxylases stand out from the ones found in other species: they are far more effective, in addition to being more versatile and resistant to chemical conditions, as tested in coordination by a Ph.D. student, Ms. Tayssir Kadri.

To test the microscopic cleaner, the research team purified a few of the enzymes and used them to treat samples of contaminated soil. "The degradation of hydrocarbons using the crude enzyme extract is really encouraging and reached over 80% for various compounds," said Brar.

The process is effective in removing benzene, toluene, and xylene, and has been tested under a number of different conditions to show that it is a powerful way to clean up polluted land and marine environments."

The next steps for Brar's team are to find out more about how these bacteria metabolize hydrocarbons and explore their potential for decontaminating sites. One of the advantages of the approach developed at INRS is its application in difficult-to-access environments, which present a major challenge during oil spill cleanup efforts.

Research paper


Related Links
Institut national de la recherche scientifique - INRS
All About Oil and Gas News at OilGasDaily.com


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OIL AND GAS
Riyadh: No major damage to oil tanker after Yemeni attack
Washington (UPI) Apr 4, 2018
An oil tanker moving through the Red Sea suffered only minor damage after it was attacked by Houthi rebels in Yemen, the Saudi government said. Col. Turku al-Maliki, spokesman for the Saudi-led coalition in Yemen, said on Twitter that naval forces were able to thwart a major escalation after Houthi rebels targeted a Saudi oil tanker. Speaking through the official Saudi Press Agency, the spokesman said the attack was in international waters and a flagrant violation to maritime rules in th ... read more

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